Studies on Monogenetic Trematodes. XV. Dactylogyridae from Alaska, Wisconsin, and Wyoming

Mizelle, John; Webb, Francis

Available specimens up to a maximum of twenty were used in the treatment of each species. Only two Tetraonchinae namely, Tetraonchus variabilis from the round white fishes Prosopium w. williamsoni and Prosopium cylindraceum quadrilaterale, and T. rauschi from the grayling, Thymallussignifer, are new. The host distribution of the remaining nine species is as follows: Cleidodiscus floridanus and C. pricei from the channel cat, lctalurus l. lacustris; C. vancleavei from the white crappie, Pomoxis annularis; Murray-tremacopulata from the rosyside sucker, Catostomus fecundus; Urocleidus chrysops and U. mimus from the white bass, Lepibema chrysops; Dactylogyrus bulbus and D. cornutus from the common shiner, Notropis cornutus frontalis; and D. extensus from the carp, Cyprinus carpio. The host material was first frozen and then preserved in five per centformalin. The gills were placed in small vials about two thirds full of tap water and shaken vigorously in order to free the parasites from the branchial tissue. This material was then transferred to Syracuse watch glasses and diluted and decanted until sufficiently clear for reliable examination with a dissectoscope. Specimens were transferred to clear water for removal of excess mucus. Each permanent mount was made by selecting a single specimen microscopically with a capillary pipette and transferring it to a drop of solidified glycerin-gelatin medium on a clean glass slide. A cover glass which was placed thereon settled in place as the medium was melted with gentle heat. The slide was then cooled, the medium congealed, and a permanent mount was thus produced.

Studies on Monogenetic Trematodes. XV. Dactylogyridae from Alaska, Wisconsin, and Wyoming

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