Caldicellulosiruptor obsidiansis sp. nov., an Anaerobic, ExtremelyThermophilic, Cellulolytic Bacterium Isolated from ObsidianPool, Yellowstone National Park
Hamilton-Brehm SD, Mosher JJ, Vishnivetskaya T, Podar M, Carroll S, Allman S, Phelps TJ, Keller M, Elkins JG. 2010. Caldicellulosiruptor obsidiansis sp. nov., an Anaerobic, ExtremelyThermophilic, Cellulolytic Bacterium Isolated from ObsidianPool, Yellowstone National Park. Applied and Environmental Microbiology. 76(4):1014-1020
A novel, obligately anaerobic, extremely thermophilic, cellulolytic bacterium, designated OB47T, was isolatedfrom Obsidian Pool, Yellowstone National Park, WY. The isolate was a nonmotile, non-spore-forming, Grampositiverod approximately 2 m long by 0.2 m wide and grew at temperatures between 55 and 85°C, with theoptimum at 78°C. The pH range for growth was 6.0 to 8.0, with values of near 7.0 being optimal. Growth oncellobiose produced the fastest specific growth rate at 0.75 h1. The organism also displayed fermentativegrowth on glucose, maltose, arabinose, fructose, starch, lactose, mannose, sucrose, galactose, xylose, arabinogalactan,Avicel, xylan, filter paper, processed cardboard, pectin, dilute acid-pretreated switchgrass, andPopulus. OB47T was unable to grow on mannitol, fucose, lignin, Gelrite, acetate, glycerol, ribose, sorbitol,carboxymethylcellulose, and casein. Yeast extract stimulated growth, and thiosulfate, sulfate, nitrate, andsulfur were not reduced. Fermentation end products were mainly acetate, H2, and CO2, although lactate andethanol were produced in 5-liter batch fermentations. The GC content of the DNA was 35 mol%, and sequenceanalysis of the small subunit rRNA gene placed OB47T within the genus Caldicellulosiruptor. Based on itsphylogenetic and phenotypic properties, the isolate is proposed to be designated Caldicellulosiruptor obsidiansissp. nov. and OB47 is the type strain (ATCC BAA-2073).
- Type
- Journal Article
- Authors
- Hamilton-Brehm, Scott; Mosher, Jennifer; Vishnivetskaya, Tatiana; Podar, Mircea; Carroll, Sue; Allman, Steve; Phelps, Tommy; Keller, Martin; Elkins, James
- Units
- GRYN
- Keywords
- fermentative growth, nonmotile, non-spore-forming, obligately anaerobic