Detection of infectious tomato mosaic tobamovirus in fog and clouds
Castello J, Lakshman D, Tavantzis S, Rogers S, Bachand G, Carlisle J, Liu Y. 1995. Detection of infectious tomato mosaic tobamovirus in fog and clouds. Ecology and Epidemiology: The American Phytopathological Society. 85(11):1409-1412
This article investigates the potential for the atmospheric spread of plant viruses. 'We contend that virus incidence is related to exposure of spruce to virus-laden clouds or fog.''Tomato mosaic tobamovirus (ToMV) was detected by enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction-bolt hybridization (RT-PCR-BH) in cloud samples collected from the summit of Whiteface Mountain, NY, and in fog samples from two collection sites along the coast of Maine. The virus was subsequently transmitted to Chenopodium quinoa from a composite of RT-PCR-BH-positive concentrates. There was no apparent relationship between the presence of ToMV and sample collection date, volume, or pH. The RT-PCR products of three cloud and fog samples and one deionized water sample were identical to one another and to a stream water isolate of ToMV from Whiteface Mountain based on nucleotide sequencing of a 347-bp fragment within the coast protein gene and 99.1 and 72.9% similarity to ToMV-L and tobacco mosaic virus-vulgare, respectivley' GIS Coordiantes for fog or cloud sampling locations is provided. /STUDY LOCATIONS:/ /ISLE AU HAUT/MOUNT DESERT ROCK/
- Type
- Journal Article
- Authors
- Castello, J.D.; Lakshman, D.K.; Tavantzis, S.M.; Rogers, S.O.; Bachand, G.D.; Carlisle, J.; Liu, Y.
- Units
- ACAD
- Keywords
- acid fog, Atmosphere, Clouds, Disease, fog, Forest, Isle Au Haut, Mount Desert Rock, Tomato Mosaic Tobamovirus, virus, waterborne virus.