Taxonomy of Douglas fir (Pseudotsuga menziesii) infraspecific taxa: vars. menziesii, glauca and oaxacana: nrDNA, and cpDNA sequences and leaf essential oils
Adams RP, Vargas-Hernandez JJ, Elizondo MG, Hunter G, Fairhall TA, Thornburg D, Callahan F. 2013. Taxonomy of Douglas fir (Pseudotsuga menziesii) infraspecific taxa: vars. menziesii, glauca and oaxacana: nrDNA, and cpDNA sequences and leaf essential oils. Phytologia. 95(1):1-9
Six chloroplast DNA regions of Douglas fir (Pseudotsuga menziesii) from 11 populations from Washington southward to Oaxaca, Mexico were sequenced yielding 4857 bp of data as well as sequences for nrDNA from P. macrocarpa, P. m. var. menziesii, (3 populations) and var. glauca (partial, NM population). Pseudotsuga macrocarpa was included as an outgroup. The nrDNA grouped the three var. menziesii separate from var. glauca (NM), even with only partial sequences. Very little variation was found among the populations of P. menziesii but the cpDNA did give support for the recognition of var. menziesii and var. glauca, with some support for var. oaxacana. However, for the cpDNA data, the population of P. menziesii var. menziesii on serpentine soil in sw Oregon had its highest affinity to var. glauca in Arizona. In contrast, the previous study using terpenoids (Adams et all 2012) clearly placed the serpentine soil Oregon trees with var. menziesii. The Oregon population may be introgressed by the chloroplast from inland (var. glauca) germplasm, leading to these results. The var. oaxacana differed by 3 MEs (mutational events) from var. glauca, and by 2 MEs from a tree in nearby El Chico NP, Hgo. Pseudotsuga menziesii var. glauca appears very uniform for these cpDNA markers from Wyoming to Arizona, New Mexico and into northern Mexico. The terpenoid data seems to reflect the status of current evolution and the cpDNA data indicates ancestral relationships.
- Type
- Journal Article
- Authors
- Adams, Robert; Vargas-Hernandez, J.; Elizondo, M. Socorro; Hunter, Gay; Fairhall, Thomas; Thornburg, David; Callahan, Frank
- Units
- OLYM
- Keywords
- DNA sequences, Douglas Fir, P. menziesii var. glauca, P. menziesii var. oaxacana, Pseudotsuga menziesii var. menziesii, Taxonomy