Novel tetranucleotide microsatellite DNA markers for the wood frog, Rana sylvatica
Julian SE and King TL. 2003. Novel tetranucleotide microsatellite DNA markers for the wood frog, Rana sylvatica. Molecular Ecology Notes. 3(2):256–258
Fifteen tetranucleotide microsatellite loci were identified and characterized for wood frogs (Rana sylvatica) collected from three vernal pools in the southeastern US. These markers revealed a high degree of genetic diversity (nine to 34 alleles per locus), heterozygosity (30.6–92.3%) and allelic heterogeneity (69% of comparisons were statistically significant). Considerable differentiation among populations was observed as genetic distances (chord) ranged between 0.40 and 0.55 and all FST values (0.02–0.05) were statistically significant. Genotypic assignment tests correctly classified 103 of 113 individuals to their respective collection. These markers should prove useful for investigating fine-scale population structure and metapopulation dynamics.
- Type
- Journal Article
- Authors
- Julian, Shannon; King, Tim
- Units
- GRSM
- Keywords
- Bote Mountain, Cades Cove, GRSM-00031, microsatellite DNA, Population Structure, wood frog
- Subjects
- Ecological Framework: Biological Integrity | Focal Species or Communities | Amphibians and Reptiles