Yellowstone Lake Nanoarchaeota
Clingenpeel S, Kan J, Macur RE, Woyke T, Lovalvo D, Nealson K, Mcdermott TR. 2013. Yellowstone Lake Nanoarchaeota. Frontiers in Microbiology. 4
ConsiderableNanoarchaeotanoveltyanddiversitywereencounteredinYellowstoneLake,YellowstoneNationalPark(YNP),wheresamplingtargetedlakefloorhydrothermalventfluids,streamersandsedimentsassociatedwiththesevents,andinplanktonicphoticzonesinthreedifferentregionsofthelake.Significanthomonucleotiderepeats(HR)wereobservedinpyrosequencereadsandinnearfull-lengthSangersequences,averaging112HRper1349bpcloneandcouldconfounddiversityestimatesderivedfrompyrosequencing,resultinginfalsenucleotideinsertionsordeletions(indels).However,SangersequencingoftwodifferentsetsofPCRclones(110bp,1349bp)demonstratedthatatleastsomeoftheseindelsarereal.ThemajorityoftheNanoarchaeotaPCRampliconswereventassociated;however,curiously,onerelativelysmallNanoarchaeotaOTU(71pyrosequencingreads)wasonlyfoundinphoticzonewatersamplesobtainedfromaregionofthelakefurthestremovedfromthehydrothermalregionsofthelake.ExtensivepyrosequencingfailedtodemonstratethepresenceofanIgnicoccuslineageinthislake,suggestingtheNanoarchaeotainthisenvironmentareassociatedwithnovelArchaeahosts.Definedphylogroupsbasedonnearfull-lengthPCRclonesdocumentthesignificantNanoarchaeota16SrRNAgenediversityinthislakeandfirmlyestablishaterrestrialcladedistinctfromthemarineNanoarcheotaaswellasfromothergeographicallocations.
- Type
- Journal Article
- Authors
- Clingenpeel, Scott; Kan, Jinjun; Macur, Richard; Woyke, Tanya; Lovalvo, Dave; Nealson, Kenneth; Mcdermott, Timothy
- Units
- GRYN , YELL
- Keywords
- Keywords: Nanoarchaeota, pyrosequencing, YellowstoneLake