Isolation and characterization of microsatellite markers in the rare clonal plant, Spiraea virginiana (Rosaceae)
Brzyski JR. 2010. Isolation and characterization of microsatellite markers in the rare clonal plant, Spiraea virginiana (Rosaceae). American Journal of Botany. 97(4):e20-e22
• Premise of the study: Microsatellite markers were developed in Spiraea virginiana , a federally threatened native shrub found along stream banks, to identify clonal genotypes and measure population genetic variability. • Methods and Results: Eleven primer sets were developed using a non-radioactive protocol. These revealed a moderate level of genetic variation, as indicated by the number of alleles per locus (range = 1 – 4) and an average observed heterozygosity of 0.595. Select loci also amplifi ed successfully in the related species Spiraea japonica. • Conclusion: Development of the markers described here is critical for the genetic identifi cation of clonal plants as a fi rst step in demographic analyses, and is necessary for the future conservation of this rare species. Amplifi cation of the markers in S. japonica suggests their potential utility in research regarding this species.
- Type
- Journal Article
- Authors
- Brzyski, Jessica
- Units
- GRSM
- Keywords
- clonal, GRSM-00899, microsatellite, Rosaceae, Spiraea virginiana
- Subjects
- Ecological Framework: Biological Integrity | At-risk Biota | T&E Species and Communities