Preparation of Single-Celled Marine Dinoflagellates for Electron Microscopy
Truby EW. 1997. Preparation of Single-Celled Marine Dinoflagellates for Electron Microscopy. Microscopy Research and Technique. 36(4):337-340
Electron microscopy has been used successfully to study and identify single-celled marine dinoflagellates including parasitic ones and others, such as those that cause red tide. Delicate cells can be preserved for scanning electron microscopy with a combined glutaraldehydeosmium tetroxide mixture that is adjusted for the osmolality of the medium. The protocol allows resolution of fine morphological features. Preservation for transmission electron microscopy can be accomplished with a standard glutaraldehyde fixation and osmium-tetroxide post-fixation in a suitable buffer, but again, the osmolality of the mixture must be adjusted. The protocol allows ultrastructural resolution of vesiculated cells and has been modified for small sample sizes.
- Type
- Journal Article
- Authors
- Truby, Earnest
- Units
- SFCN
- Keywords
- Armored, Flagellum, Morphology, Osmolality, Unarmored