Separation of Ectoderm and Endoderm from Sea Urchin Pluteus Larvae and Demonstration of Germ Layer-Specific Antigens
McClay D and Marchase R. 1979. Separation of Ectoderm and Endoderm from Sea Urchin Pluteus Larvae and Demonstration of Germ Layer-Specific Antigens. Developmental Biology. 71:289 - 296
A simple method is described for the separation of ectoderm from endoderm in sea urchin pluteus larvae. The separation, by sequential treatments with glycine-EDT A, permits an examination of the basement membrane beneath the ectoderm layer and of a fibrous layer beneath the basement membrane. In a serological study the two germ layers are shown to differ with respect to cell surface antigen composition. Further, the basement membrane is shown to contain ectoderm antigens.
- Type
- Journal Article
- Authors
- McClay, David; Marchase, Richard
- Units
- SFCN
- Keywords
- Genetics, Physical, Physiology, sea urchin