Molecular Detection of the Causative Agent of White-nose Syndrome on Rafinesque’s Big-eared Bats (Corynorhinus rafinesquii ) and Two Species of Migratory Bats in the Southeastern USA
Bernard RF, Foster JT, Willcox EV, Parise KL, Mccracken GF. 2015. Molecular Detection of the Causative Agent of White-nose Syndrome on Rafinesque’s Big-eared Bats (Corynorhinus rafinesquii ) and Two Species of Migratory Bats in the Southeastern USA. Journal of Wildlife Diseases. 51(2)
Pseudogymnoascus destructans, the causal agent of white-nose syndrome (WNS), is responsible for widespread mortality of hibernating bats across eastern North America. To document P. destructans exposure and infections on bats active during winter in the southeastern USA, we collected epidermal swabs from bats captured during winters 2012–13 and 2013–14 in mist nets set outside of hibernacula in Tennessee. Epidermal swab samples were collected from eight Rafinesque’s big-eared bats (Corynorhinus rafinesquii), six eastern red bats (Lasiurus borealis), and three silver-hair bats (Lasionycteris noctivagans). Using real-time PCR methods, we identified DNA sequences of P. destructans from skin swabs of two Rafinesque’s big-eared bats, two eastern red bats, and one silver-haired bat. This is the first detection of the WNS fungus on Rafinesque’s big-eared bats and eastern red bats and the second record of the presence of the fungus on silver-haired bats.
- Type
- Journal Article
- Authors
- Bernard, Riley; Foster, Jeffrey; Willcox, Emma; Parise, Katy; Mccracken, Gary
- Units
- GRSM
- Keywords
- Bats, eastern red bat, Fungus, Pseudogymnoascus destructans, Rafinesque’s big-eared bat, silver-haired bat, swabs, White-Nose Syndrome, Winter
- Subjects
- Ecological Framework: Biological Integrity | Infestations and Disease | Animal Diseases , Ecological Framework: Biological Integrity | Focal Species or Communities | Mammals