Isolation and Identification of Antimicrobial-Producing Microbes from Soil Associated with an Eastern Hemlock in the Great Smoky Mountains National Park
Wilson M. 2013. Isolation and Identification of Antimicrobial-Producing Microbes from Soil Associated with an Eastern Hemlock in the Great Smoky Mountains National Park. University of North Carolina. Asheville, NC
ABSTRACT: This research focused on the isolation and identification of antimicrobial-producing microbes from the Great Smoky Mountains National Park. Oligotrophic media was used to culture all microbial isolates. Antimicrobial screening was then performed by subjecting each soil isolate to potentially pathogenic microorganisms on nutrient agar test plates with growth of pathogens being inhibited if antimicrobial compounds were secreted into the culture medium. Select soil isolates that tested positive for antimicrobial activity were identified by analysis of sequences of small subunit rRNA genes following amplification by PCR. Antimicrobial production by Nocardia grenadensis strain X0885 isolated from soil obtained from the park has not been previously described.
- Type
- Unpublished Report
- Authors
- Wilson
- Date of Issue
- 2013-05
- Publisher
- University of North Carolina
- Units
- GRSM
- Keywords
- All Taxa Biodiversity Inventory, antimicrobial, APHN, Appalachian Highlands Network, ATBI, eastern hemlock, Great Smoky Mountains National Park, GRSM, GRSM-00405, microbes, Nocardia grenadensis, SER, soils, Southeast Region, Tsuga canadensis