Oak-leaf-litter rhizomorphs from Iowa and Texas: Calcium oxalate producers

Horner, Harry; Tiffany, Lois; Knaphus, George

Unidentified basidiomycete rhizomorphs growing on oak-leaf litter (Quercus alba) in Iowa and in Texas (Quercus gravesii displayed arrays of crystals associated with their hyphae. X-ray diffraction and birefringence analyses identified the crystals as a mixture of calcium oxalate-monohydrate and -dihydrate. The Iowa oak-leak-litter rhizomorph crystals occurred in two forms: young hyphae displayed either small styloid-like crystals oriented in all directions along the hyphae; or large clusters of elongated styloid-like crystals surrounding the hyphae, with individual crystals in each cluster displaying pyramidal ends. Crystals associated with the Texas oak-leaf-litter rhizomorphs consistently covered all of the young hyphae and their tips with either small dagger-like crystals or thin, plate-like crystals whose margins were either smooth or finger-like. Some larger crystal masses were also composed of crystals with pyramidal ends. The dagger-like and plate-like crystals were tentatively identified as the monohydrate from based on their higher birefringence, whereas the crystals with pyramidal ends were identified as the dihydrate form based on their shape and lower birefringence. It is not known whether the two crystalline forms associated with the rhizomorphs are a function of the individual rhizomorphs, the litter source, the stage of crystal growth, or the ions present in the surrounding soil/ground water.

Oak-leaf-litter rhizomorphs from Iowa and Texas: Calcium oxalate producers

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